Volume 4, Number 1 (2019)
Year Launched: 2016
Journal Menu
Archive
Previous Issues
Why Us
-  Open Access
-  Peer-reviewed
-  Rapid publication
-  Lifetime hosting
-  Free indexing service
-  Free promotion service
-  More citations
-  Search engine friendly
Contact Us
Email:   service@scirea.org
Home > Journals > SCIREA Journal of Clinical Medicine > Archive > Paper Information

Fingerprint identification of p. notoginseng flower, p. ginseng flower and p. quinquefolium lower based by HPLC and NIRS

Volume 4, Issue 1, February 2019    |    PP. 11-30    |PDF (1033 K)|    Pub. Date: March 23, 2019
73 Downloads     313 Views  

Author(s)
Huang Z.Q., Wenshan College, Wenshan 663000, China; Wenshan biological resources development research center, Wenshan 663000, China; Yunnan province Sanqi Key Laboratory, kunming 650000, China
Gao M.J., Wenshan College, Wenshan 663000, China; Wenshan biological resources development research center, Wenshan 663000, China; Yunnan province Sanqi Key Laboratory, kunming 650000, China
Feng G.Q, Wenshan College, Wenshan 663000, China
Ma X.S, Wenshan College, Wenshan 663000, China
Wang R, Wenshan College, Wenshan 663000, China

Abstract
Abstract: Aims: In view of the current study on the fingerprint of panax pseudo-ginseng, panax ginseng and panax quinquefolium, the identification features can not be clearly distinguished. HPLC and NIRS analysis technologies were used to establish the fingerprint of p. notoginseng flower, p. ginseng flower and p. quinquefolium flower, and to compare and analyze the differences among them. Methods: By comparing the retention time of reference substances peaks, identified peaks, established fingerprints, and analyzed and compared the different characteristics of fingerprint of each variety. The WinISI II NIRS analysis software was used to establish the original NIRS fingerprint map of each variety, the first-order and the second-order derivative processing fingerprint map, to analyze and compare the different characteristics of the fingerprint map of each variety. Results: Six peaks of p. notoginseng flower Re, R1, Rb1, Rc, Rb3 and Rd were identified, five peaks of p. ginseng flower Re, R1, Rb1, Rc and Rd were identified, and five peaks of panax quinquefolium Re, Rb1, Rc, Rb3 and Rd were identified. p. notoginseng flower (S) and p. ginseng flower (R) features of HPLC fingerprint differences mainly in peak S16, S20, S22, S23, S30, S31, S32. HPLC fingerprint characteristics of p. notoginseng flower (S) and p. quinquefolius flower (X) were mainly in peak S16, X13, X14, X23, X24, X25, X35 X36, X40. HPLC fingerprint features of p. ginseng flowers and p. quinquefolius were mainly characterized by peak R27, X20, X21, X34, X36, X37, X38. The absorption peaks of p. notoginseng flower NIRS original fingerprint at 2000~2200 nm were different from others. The first-derivative fingerprint of p. notoginseng flower and p. quinquefolium flower had peaks between 2300~2350 nm, while the p. ginseng flower were not.The peaks of p. ginseng flowers and American ginseng flowers between 2090~2150 nm, p. notoginseng flowers were without. The peaks between 1670~1690 nm of the fingerprint of p. notoginseng flower were different from others. The second derivative of p. notoginseng at 2250~2300 nm were different from others, and there were not difference at 2300~2350 nm between p. notoginseng flower and p. quinquefolium flower, while different from p. ginseng flowers. Conclusion: HPLC and NIRS fingerprints of the flower of p. notoginseng, p. ginseng and p. quinquefolium could be used to distinguish and identify them. HPLC fingerprint were identified remarkable and NIRS fingerprint could quickly identify them with zero damage.The fingerprint identification method established in this paper had the advantages of obvious features and shortened analysis time.It provided a reliable reference for further identification and research and development of p. notoginseng flower, p. ginseng flower and p. quinquefolium flower.

Keywords
Keywords: p. notoginseng flower; p. ginseng flower; p. quinquefolium flower; HPLC fingerprint; NIRS fingerprint

Cite this paper
Huang Z.Q., Gao M.J., Feng G.Q, Ma X.S, Wang R, Fingerprint identification of p. notoginseng flower, p. ginseng flower and p. quinquefolium lower based by HPLC and NIRS, SCIREA Journal of Clinical Medicine. Vol. 4 , No. 1 , 2019 , pp. 11 - 30 .

References

[ 1 ] Yang guang, Cui xiu-ming, Chen min, et al. Study on ingredients of sanqi stem and leaf and sanqi flower new food [J]. Chinese journal of pharmacy, 2007, 52 (7) : 543-547.
[ 2 ] Liu zhao, Zhong Li juan. Identification and application analysis of Chinese herbal medicine p. notoginseng flowers [J]. Medicine, 2016(7):00184-00184.
[ 3 ] Li jin-hua, Chen chun-nan, Gu cai-mei, et al. Identification of p. quinquefolium and p. quinquefolium by nonlinear chemical fingerprint technique [J]. Journal of pharmacy, 2017 (7) : 1150-1156 .
[ 4 ] Meng xiang-song, Jiang lei, Yu xian-hua, et al. HPLC fingerprint identification of sanqi flower and its confusable products [J]. Journal of liaoning university of traditional Chinese medicine, 2018 (6) .
[ 5 ] Yang wen-zhi, Du yue-zhong,Gao yu, et al. Experimental study on the identification of ginseng flowers by rp-hplc[J]. Ginseng research, 2016, 28 (6) : 13-17.
[ 6 ] Huang ya-wei, Wang jia-hua, Li xiao-yun, et al. Rapid identification of p. quinquefolium based on near-infrared spectroscopy [J]. Spectroscopy and spectral analysis, 2010, 30 (11) : 2954-2957.

Submit A Manuscript
Review Manuscripts
Join As An Editorial Member
Most Views
Article
by Sergey M. Afonin
2916 Downloads 18864 Views
Article
by Syed Adil Hussain, Taha Hasan Associate Professor
2271 Downloads 15656 Views
Article
by Omprakash Sikhwal, Yashwant Vyas
2345 Downloads 14039 Views
Article
by Munmun Nath, Bijan Nath, Santanu Roy
2245 Downloads 13935 Views
Upcoming Conferences