5-tRF-His, tRNA-derived fragments, regulate CKAP2 to inhibit the proliferation of breast cancer

Volume 9, Issue 1, February 2024     |     PP. 8-29      |     PDF (4467 K)    |     Pub. Date: May 10, 2020
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Author(s)

Xun Tang, Department of Clinical Laboratory, the Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Baiziting Road 42, Nanjing 210009, P. R. China.
Pan Jiang, Department of Clinical Laboratory, the Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Baiziting Road 42, Nanjing 210009, P. R. China.
Huanhuan Chen, Department of Clinical Laboratory, the Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Baiziting Road 42, Nanjing 210009, P. R. China.
Jun Wu, Department of Clinical Laboratory, the Affiliated Brain Hospital of Nanjing Medical University, Guangzhou Road 264, Nanjing 210009, P. R. China.
Xiaodong Xie, Department of Radiology, the Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Baiziting Road 42, Nanjing 210009, P. R. China.
Xuelian Mao, Department of Clinical Laboratory, the Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Baiziting Road 42, Nanjing 210009, P. R. China.
Dongping Mo, Department of Clinical Laboratory, the Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Baiziting Road 42, Nanjing 210009, P. R. China.
Li Tang, Department of Clinical Laboratory, the Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Baiziting Road 42, Nanjing 210009, P. R. China.
Feng Yan, Department of Clinical Laboratory, the Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Baiziting Road 42, Nanjing 210009, P. R. China.

Abstract
tRNA derived fragments are differentiated expressed in human breast cancer( BC) tissues. Among of them, 5-tRF-His attracted our attention due to its potential role in breast cancer progression. PCR and in situ hybridization were used to measure the expression and location of 5-tRF-His. The role of 5-tRF-His in vitro was explored by cell proliferation assays and flow cytometery. The effect of 5-tRF-His in vivo was performed by Xenograft experiments and immunohistochemistry. We determined the derivation of 5-tRF-His by northern blot and clarified the mechanism of 5-tRF-His by immunoprecipitation and western blot. We observed that strong downregulation of 5-tRF-His in clinical BC samples. Reduced 5-tRF-His can lead to lymph node metastasis. 5-tRF-His, a tRNA-derived fragment linked to pan-ago, regulates the proliferation and apoptosis of BC cells and inhibits BC tumor growth explant tumor in vivo. The increase in proliferation and the decrease in apoptosis induced by the 5-tRF-His inhibitor were reversed by si-CKAP2. 5-tRF-His inhibits BC development in vivo and vitro. The 5-tRF-His/CKAP2/erk2 axis affects the proliferation and apoptosis of BC cells, imply that 5-tRF-His may be a promising tumor marker of BC patients serum and treatment target for BC therapy.

Keywords
breast cancer; serum; tRNA-derived fragments; CKAP2; proliferation; ERK2 signaling pathway

Cite this paper
Xun Tang, Pan Jiang, Huanhuan Chen, Jun Wu, Xiaodong Xie, Xuelian Mao, Dongping Mo, Li Tang, Feng Yan, 5-tRF-His, tRNA-derived fragments, regulate CKAP2 to inhibit the proliferation of breast cancer , SCIREA Journal of Clinical Medicine. Volume 9, Issue 1, February 2024 | PP. 8-29.

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